Image Analysis using Z-LaP Tracker
This tutorial will guide the user through analyzing the images (1) obtained from imaging zebrafish larvae in 96-well plates.
- Obtained through our Image Acquisition tutorial.
This tutorial is only applicable for Brown University members, as it requires the use of OSCAR, Brown University's high performance computing cluster. All other users are free to use our publicly available implementation on GitHub, which includes local installation instructions and use guides.
Materials
If you are doing this protocol for the first time, ask our resident Research Assistant to help you set up account(s) and permissions.
Setting up Globus
What is Globus?
Globus is a cyberinfrastructure used in research to move and share data between users, computing clusters, and even organizations while protecting the security and integrity of the data. Globus allows us to seamlessly move data between the R-Drive and OSCAR for image analysis.
Running Analysis on Oscar
What is Oscar?
Oscar stands for Ocean State Center for Advanced Resources. It is Brown University's high performance computing cluster and allows users to run resource-intensive "jobs" and processes (e.g. scripts, analysis, inference, etc.) that would take significantly longer on a personal computer.