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Welcome

This is the Creton lab's page for protocols and other resources. For a list of current projects please visit our github page.

What we do

Our work focuses on investigating the behavior of zebrafish larvae exposed to a variety of compounds. We are continuously developing new tools for behavioral acquisition, analysis, and quantification.

Zebrafish

384-well set up

We currently have two methods for the analysis of four 96-well plates:

  • ImageJ/Fiji - A custom-developed macro that uses a substraction method to distinguish the larvae from the background. The positions of the larvae are stored in an Excel file.
  • Z-LaP Tracker - A model trained using the markerless position estimation software DeepLabCut. The positions of the larvae are stored in a CSV file.

For a complete description of these methods, please refer to our previous work on the screening of FDA-approved compounds1 and the development of Z-LaP Tracker 2.

Mice

Coming soon

  1. Tucker Edmister, Sara et al. “Novel use of FDA-approved drugs identified by cluster analysis of behavioral profiles.” Scientific reports vol. 12,1 6120. 21 Apr. 2022, doi:10.1038/s41598-022-10133-y ↩

  2. Gore, Sayali V et al. “Zebrafish Larvae Position Tracker (Z-LaP Tracker): a high-throughput deep-learning behavioral approach for the identification of calcineurin pathway-modulating drugs using zebrafish larvae.” Scientific reports vol. 13,1 3174. 23 Feb. 2023, doi:10.1038/s41598-023-30303-w ↩